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How to choose a Raman microscope?
ATR8XXX is the Raman microscope series including:
ATR8300 series is available single/dual-band
ATR8500 is available in single/dual/triple-band.
ATR8800 is scientific-grade Raman microscope available up to quadriband.
Q: How to choose wavelengths of Raman Spectrometer?
266nm wavelength can differ the fluorescence signal from Raman signals so that it can be used to measure fluorescent materials
523nm wavelength is usually used to detect graphene, virus.
633nm wavelength is suitable for metallic oxide.
785nm wavelength can satisfy the general-purpose measurement
830nm wavelength is suitable for noninvasive blood glucose.
1064nm wavelength is suitable for the biological tissue, cell, bacteria, fuel, and high fluorescence samples.
What is Raman Microscope?
Optosky Raman microscope integrates microscope and Raman spectrometer. It sees micro areas of samples on the screen in a single click. Once one accurate location is visualized, the observer can detect Raman signals under various surface conditions, and synchronized Mapping image can be displayed intuitively on the screen at one click, result in great convenience to detect micro or macro areas of samples. Combine true confocal system with accurate image process algorithm, and a small sample area can be analyzed, as well as it requires minimal training and maintenance, yet resulting in a uniform result.
The microscope is equipped with a tailor-made objective, and laser spot on the sample becomes very close to the diffraction limit, then focal information can be displayed accurately and intuitively on the screen with a 3-megapixel camera. Its advantage improves Raman spectral quality and overcomes the limitations of Raman systems where the focal plane for Raman signal acquisition is little above or below the imaging plane.
It works stably without moving components of optical path switch, hence it avoids optical path loss while the image formed, and it gains an optimized signal by separating the image formed through Raman signal acquisition.
What is Raman microscope used for?
Raman microscope is used for nanoparticles and new materials, Nanoparticles and new materials, science research, bioscience, forensic, medical immunology analysis, agriculture, environmental science etc.
What does Raman microscope tell you?
Raman mircoscope can measure new materials ID,nano materials ID, disease, virus, microbe, bateria etc.
How much does a Raman microscope cost?
Optosky Raman spectrometer prices range from USD39,800 to USD99,980 above.
How does Raman microscope work?
Raman microscope is made up of microscope,microspectrometer,lasers, integrated Raman probe and software,monochromic light is generated by laser shoot on the surface of sample happening Raman scattering, scattering light goes through Raman probe return to optic fiber spectrometer. System focus automatically looks for the best location to detect, readout spectra of the sample via Optosky software.
Where to buy Optosky Raman microscope?
Optosky portable dual-band Raman spectrometer can be bought from local distributors, order online or contact online sales.
Why choose Optosky Raman microscope?
Optosky has been developing Raman spectrometer for 20-year.
Optosky is China National Standards drafter of Raman spectrometer
Top 1 in China brand and Top 3 brand in the world.
In 2016 launch the first generation handheld Raman spectrometer,
In 2017 launch the dual-band Raman spectrometer
In 2020, launch the triple-band Raman microscope
In 2021, launch the quadriband scientific-grade Raman microscope
Optosky represents the highest performance cost less Chinese high-tech manufacturers with industry-leading warranty guarantee and technical support.
Optosky's Raman spectroscopy technology originate from Shanghai Institute of Technical Physics Chinese Academic of Science University and Xiamen University Zhong Qun Tian who discovers SHINERS makes SERS can be preserved longer than 2 years.
Quality control system follows Agilent Technologies
60% R&D engineers provide Industrial-leading warranty guarantee and technical support services
The most experienced engineers and 3 Drs team in Raman spectroscopy
Complete certification of ISO, CE, FDA Approval, Police Approval, API Approval
Q: Why the 1064nm Raman can resisit high fluorescence materials?
A: 1064nm belong to NIR photon has low frequency, low energy is seldom absorbed by the sample molecule, and it's difficult to excite ground state electron as well as fluorescence, so that it prevent fluorescence from generation.
Q: Why Raman signals are easily annihilated by fluorescence?
A: Fluorescence intensity is about 10^6 times stronger than that of raman signal. traditional Raman signals are easily annihilated by fluorescence.
Q: Which materials have high fluorescence recommended to use 1064nm Raman?
A: Such as Heroin, Colour samples, Biological samples, Textiles, Bacteria, Drugs, Fuel, Petroleum samples, Vegetable oils, Explosives, Plastic.
How many methods can remove fluorescence interference?
1st, Baseline correction use algorithm to remove fluorescence interference.
2nd, Diference Raman use two wavelengths of lasers (eg: 784.5nm+785.5nm) to excite samples can obtain two sets of Raman signal and calculate the difference to obtian the spectrum without fluorescence.
3rd, Time gate control method
4th Florescence photobleaching method
5th, 1064nm Raman spectrometer is highly recommended
Q: How is the Raman spectroscopy technology is discovered?
A: There is a very interesting story told in 1920s, an Indian scientist named Raman discover why the sea is so blue, whose blue brighter than blue sky, and after his research, he finally found in 1928 that the sea has absorbed uv range and generated new blue lights. Raman write a paper of his discovery, in order to remember Raman scientist great discovery and people use his name to describe this phenomenon Raman Spectroscopy. The first Discovery of Raman spectroscopy in 1928. And in 1930s, Raman scientist has won the Nobel prize. In the scientists history, Raman spectroscopy has been a very big discovery.
Q: What is the Raman spectroscopy working principle?
A: When the molecule absorbs laser energy and generate energy level shift, but it is an unstable state. It can ring-down to the original energy level. If no energy shift part called Rayleigh scattering. However some part shift from E0 to E2, fall down to E1, This situation is called STOCKS, a single photon energy is lower energy & frequency than the laser energy but longer wavelength. The other part from E1 to higher energy level E1+hv0, and its ring down to E0 with the higher frequency and energy, but shorter wavelength called anti-stokes lines. In these situations still ring down to the deeper energy levels but less. Raman spectroscopy are mainly analyzing the STOKES lines.
Q: How is the Raman spectrometer work?
A: First of all, the narrow linewidth laser generates an excitation light go through the fiber and collimator mirror to form a parallel incident light, excitation light focus from here, and shoot on the sample to be detected. The sample absorbs excitation light energy and it happens the energy levels shift, then go through ring-down process of generating new Raman light with a 360 degree scattering. Some portions of Raman scattering can be collected by our objective lens, then reflected from here, through long pass filter Rayleigh scattering, then couple into inside fiber and spectrometer. Raman spectrometer is used to analyze the collected Raman signal, wavelength, and intensity. Finally, it can get the Raman signal.
Q: How is the spectrometer work?
A: The CCD export signal going through circuit, DC power, and USB, then transfer on the PC software, and finally obtain spectrum result.
Q: What is the application of Raman spectrometer?
A:
1st, Biomedical industry of early cancer detection, ultrastructure change of biological molecule, calculi components analysis.
2nd, Public Safety of Drugs and Narcotics, Fast Detect Toxic Substance, explosives & Flammable materials, Drink water.
3rd, Environmental science of waste water contamination, petroleum analysis, deposits analysis.
4th, Pharmaceutical Engineer, Drugs scan and analysis, Crystallization in process monitor, Raw material QA & QC
5th, Food Safety, Detect refined gutter oil, Detect pesticides residues, Food additives and contaminants.
6th, Gemstones ID, Trace gemstones place-of-origin, Anti-counterfeiting, Antique ID
Q: What are differences between Infrared spectroscopy and Raman spectroscopy?
A: Infrared spectroscopy and Raman spectroscopy are twin brothers, their spectra are mutually complementary.
1st, Working Principle Differences:
Infrared spectroscopy absorbs molecule absorption spectra, but the Raman spectroscopy absorbs the molecular emissions spectra with higher accuracy. It’s important to get a general idea that Raman spectroscopy technology is more accurate than Infrared spectroscopy.
2nd, Infrared spectroscopy is measured by contact, most infrared measurement requires sample preparation, but Raman spectroscopy requires no sample preparation and non-contact measurement through the glasses, plastic bags, kraft papers, and envelop etc.
3rd, Measure methods are different, infrared spectroscopy is destructive measurement can damage the sample requires larger samples quantities and consumable materials, but Raman spectroscopy is non-destructive measurement and can non-contact sample, no consumables materials.
4th, Infrared spectroscopy requires sample preparation, but the Raman spectroscopy requires no sample preparation.
5th,Infrared spectroscopy measure molecular group, but Raman spectroscopy measure molecular skeleton with higher accuracy.
6th,Tell the differences of isomer, including monomer isomeride, position
7th, Infrared spectroscopy can not tell the difference of isomers, but the Raman spectroscopy can tell the difference of isomers, including monomer isomer, position isomers, geometric isomers, variants generated in varaints fast.
8th, Infrared spectroscopy can not detect sample in water solution, which is easily influenced by the water, but Raman spectroscopy has little influence on water, even if the peak of Another one is solutions. Infrared spectra is easily influenced by the water, but Raman can not be influenced by water am water has not raman signal, even at the peak of 3400 has a small curve, which have no influence on our measurement.
9th, Infrared spectroscopy is sensitive to water vapour, however, Raman spectroscopy can detect at any state anywhere.
10th, Infrared spectroscopy requires huge amount of samples to build up modeling, but it only takes 1-2mg sample to build a library.
The ATR8600 compact confocal Raman microscopy has built-in pinhole confocal, high-resolution sp..
ATRH8800 adds a microscopic hyperspectral imager to the confocal Raman microscope. The instrument is..
ATRH8500 adds a microscopic hyperspectral imager to the Raman microscope. The instrument is an advan..
This instrument is an advanced equipment that integrates micro-area Raman spectroscopy, micro-area i..
This instrument is an advanced device that integrates micro-area Raman spectroscopy, micro-area refl..
Optosky new generation ATR8700 type split confocal Raman microscopy imager equipped w..
ATR8800 C series micro-Raman spectrometer integrates up to 4 lasers and combines the advantages of b..
The ATR8800 series scientific-grade confocal Raman imaging microscope. This is a true confocal..
ATR8300TW Series integrates two lasers, microscope, and Raman spectrometer, combining w..
The ATR8300Pro series combines the advantages of microscopes and Raman spectrometers. The Raman micr..
Optosky ATR8300MP miniature Raman microscope integrates microscope and Raman spectrometer. It featur..
ATR3110XW Raman spectrometer microscope adaptation version is a breakthrough hig..
The ATR8100 series combines the advantages of microscopes and Raman spectrometers. The Micro-Ra..
ATM8600 3D Laser Scanning Microscope is self-designed to perform non-contact profile, roug..
The ATR8820 series of ultra-high-speed transient wide-field Raman spectroscopic imagers perfectly co..
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